Hy Hass series modular protein purification system

Introduction:

- Flow rate range: 0~1200mL/min customizable
- Pressure resistance: up to 40Mpa (5600psi)
- Wavelength range: 280nm/(190-500)/(195-850nm) optional
- Detection wavelength: Simultaneous detection of three wavelengths, customizable (4, full wavelength)
- Absorption range: ± 6AU
- Interface: Ethernet communication

Scope of application

Universal pilot grade protein purification system, with adjustable modules. Research and production of biological proteins, antibodies, vaccines, and other applications ranging from laboratory to industrial levels. Mainly serving major biotech companies and CRO/CDMO enterprises, providing them with comprehensive solutions for research and development as well as production.

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HassPure25 HassPure150 HassPilot300 HassPilot600 Solutions Downloads

Item	Parameter	Notes
Appearance		(W × D × H) – [mm]: 520 × 440 × 550
System Pump	Flow Rate Range: 0–25 mL/min Maximum Pressure: 30 MPa / 4200 psi Flow Repeatability: 0.25–25 mL/min, <3 MPa, 0.8–2 cP Flow Accuracy: ±1% Flow Precision: RSD <0.5% Gradient Accuracy: ±0.6% Interface: Ethernet communication Pressure Sensor: 0–20 MPa, accuracy ±0.025 MPa Cleaning: Automatic flushing	Dual-pump gradient system 25 mL/min per pump Supports constant pressure mode and pressure control mode
Mixer	Dynamic mixer: standard 1.4 mL, optional 5 mL or 10 mL	Standard 1.4 mL
UV Detector	Single-wavelength LED light source, Abs: ±6 AU	Single wavelength 280 nm Optional: 190–500 nm, 3 wavelengths Optional: 190–850 nm, 3 wavelengths Optional: 190–850 nm, 4 wavelengths
Conductivity / Temperature Monitor	Conductivity: 0–999 mS/cm Resolution: 0.001 mS/cm Accuracy: ±1.0% Automatic temperature compensation	Standard
pH Valve	pH Range: 0–14 Resolution: 0.01 Accuracy: ±0.05 7-position, 5-port valve pH electrode and restrictor can be online or bypassed	1: Restrictor → pH 2: Restrictor 3: pH 4: Bypass 5: Restrictor
Communication Interface	TCP/IP, 100 Mbps Ethernet	Standard
Accessory Kit	Tubing, fittings and accessories	Standard
Chromatography Workstation Software	Chinese / English user interface, including instrument control, method editing, data processing, report printing, user and result database, encrypted database management, peak integration, quantitative analysis, audit trail, scouting, channel scouting, method programming and 8-channel parallel operation.	Standard
Pump A Inlet Valve	Pump A inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Pump B Inlet Valve	Pump B inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Sample Pump Inlet Valve	Sample pump inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	6 channels + 1 common port high-pressure rotary valve (7 channels)
Air Sensor, Inlet A	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Air Sensor, Inlet B	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Air Sensor, Sample Pump Inlet	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Injection Valve	8-position, 6-port high-pressure rotary valve, pressure rating 30 MPa	1: Manual Load 2: Inject 3: System Pump Waste 4: Sample Pump Load 5: Direct Inject 6: Sample Pump Waste
V Valve	1: 1–2 2: 3–4 3: 1–3 4: 2–4 Pressure rating: 30 MPa	None
Column Valve	Configurable, pressure rating 30 MPa Optional: 2-column valve / 2-column valve with reverse flow / 6-column valve with reverse flow	6-column valve, 5 column flow paths, 1 bypass line
Column Delta Pressure Module	Pre-column pressure, post-column pressure and delta pressure	Standard
Outlet Valve	Maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Back Pressure Valve / Flow Restrictor	HY-FR804	Standard system back pressure: 0.3 MPa
Fraction Collector	1. Frac100A: Open-frame collector, compatible with standard XY100 tubes 2. Frac100B: Enclosed cabinet, 6 rack positions, compatible with 50 mL and 15 mL tubes, automatic rack recognition 3. Frac100B-1: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, automatic rack recognition 4. Frac100B-2: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, with UV sterilization and temperature / humidity monitoring, automatic rack recognition 5. Frac100C: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, with UV sterilization, temperature / humidity monitoring and TE cooling, automatic rack recognition	Frac100B-1
Sample Pump	Flow Rate Range: 0–100 mL/min Maximum Pressure: 10 MPa / 1400 psi Flow Repeatability: 1–100 mL/min, <3 MPa, 0.8–2 cP Flow Accuracy: ±1% Flow Precision: RSD <0.5% Gradient Accuracy: ±0.6% Interface: Ethernet communication Pressure Sensor: 0–20 MPa, accuracy ±0.025 MPa Cleaning: Automatic flushing	Standard: 100 mL/min Optional: 150 mL/min Optional: 25 mL/min
SDK & Communication Protocol	Optimized proprietary command set. Tested development libraries are provided, including .NET libraries, C/C++ DLLs and sample source code.	Suitable for secondary development.
Human-Machine Interface	Windows 7 / Windows 10 / Windows 11	Configuration: Intel i3 processor or above, memory ≥8 GB, storage ≥256 GB, display resolution 1920 × 1080
Power Supply	110–220 VAC, ±5%
Operating Temperature	-10°C to 40°C, non-condensing

Item	Parameter	Notes
Appearance		(W × D × H) – [mm]: 520 × 440 × 550
System Pump	Flow Rate Range: 0–150 mL/min Maximum Pressure: 10 MPa / 1400 psi Flow Repeatability: 1–150 mL/min, <3 MPa, 0.8–2 cP Flow Accuracy: ±1% Flow Precision: RSD <0.5% Gradient Accuracy: ±0.6% Interface: Ethernet communication Pressure Sensor: 0–20 MPa, accuracy ±0.025 MPa Cleaning: Automatic flushing	Dual-pump gradient system 150 mL/min per pump Supports constant pressure mode and pressure control mode
Mixer	Dynamic mixer: standard 1.4 mL, optional 5 mL or 10 mL	Standard 1.4 mL
UV Detector	Single-wavelength LED light source, Abs: ±6 AU	Single wavelength 280 nm Optional: 190–500 nm, 3 wavelengths Optional: 190–850 nm, 3 wavelengths Optional: 190–850 nm, 4 wavelengths
Conductivity / Temperature Monitor	Conductivity: 0–999 mS/cm Resolution: 0.001 mS/cm Accuracy: ±1.0% Automatic temperature compensation	Standard
pH Valve	pH Range: 0–14 Resolution: 0.01 Accuracy: ±0.05 7-position, 5-port valve pH electrode and restrictor can be online or bypassed	1: Restrictor → pH 2: Restrictor 3: pH 4: Bypass 5: Restrictor
Communication Interface	TCP/IP, 100 Mbps Ethernet	Standard
Accessory Kit	Tubing, fittings and accessories	Standard
Chromatography Workstation Software	Chinese / English user interface, including instrument control, method editing, data processing, report printing, user and result database, encrypted database management, peak integration, quantitative analysis, audit trail, scouting, channel scouting, method programming and 8-channel parallel operation.	Standard
Pump A Inlet Valve	Pump A inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Pump B Inlet Valve	Pump B inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Sample Pump Inlet Valve	Sample pump inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	6 channels + 1 common port high-pressure rotary valve (7 channels)
Air Sensor, Inlet A	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Air Sensor, Inlet B	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Air Sensor, Sample Pump Inlet	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Injection Valve	8-position, 6-port high-pressure rotary valve, pressure rating 30 MPa	1: Manual Load 2: Inject 3: System Pump Waste 4: Sample Pump Load 5: Direct Inject 6: Sample Pump Waste
V Valve	1: 1–2 2: 3–4 3: 1–3 4: 2–4 Pressure rating: 30 MPa	None
Column Valve	Configurable, pressure rating 30 MPa Optional: 2-column valve / 2-column valve with reverse flow / 6-column valve with reverse flow	6-column valve, 5 column flow paths, 1 bypass line
Column Delta Pressure Module	Pre-column pressure, post-column pressure and delta pressure	Standard
Outlet Valve	Maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Back Pressure Valve / Flow Restrictor	HY-FR802	Standard system back pressure: 0.3 MPa
Fraction Collector	1. Frac100A: Open-frame collector, compatible with standard XY100 tubes 2. Frac100B: Enclosed cabinet, 6 rack positions, compatible with 50 mL and 15 mL tubes, automatic rack recognition 3. Frac100B-1: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, automatic rack recognition 4. Frac100B-2: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, with UV sterilization and temperature / humidity monitoring, automatic rack recognition 5. Frac100C: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, with UV sterilization, temperature / humidity monitoring and TE cooling, automatic rack recognition	Frac100B-1
Sample Pump	Flow Rate Range: 0–150 mL/min Maximum Pressure: 10 MPa / 1400 psi Flow Repeatability: 1–150 mL/min, <3 MPa, 0.8–2 cP Flow Accuracy: ±1% Flow Precision: RSD <0.5% Interface: Ethernet communication Pressure Sensor: 0–20 MPa, accuracy ±0.025 MPa Cleaning: Automatic flushing	Standard: 150 mL/min Optional: 100 mL/min Optional: 25 mL/min
SDK & Communication Protocol	Optimized proprietary command set. Tested development libraries are provided, including .NET libraries, C/C++ DLLs and sample source code.	Suitable for secondary development.
Human-Machine Interface	Windows 7 / Windows 10 / Windows 11	Configuration: Intel i3 processor or above, memory ≥8 GB, storage ≥256 GB, display resolution 1920 × 1080
Power Supply	110–220 VAC, ±5%
Operating Temperature	-10°C to 40°C, non-condensing

Item	Parameter	Notes
Appearance		(W × D × H) – [mm]: 520 × 440 × 550
System Pump	Flow Rate Range: 0–300 mL/min Maximum Pressure: 3 MPa / 420 psi Flow Repeatability: 3–300 mL/min, <3 MPa, 0.8–2 cP Flow Accuracy: ±2% Flow Precision: RSD <0.5% Gradient Accuracy: ±0.6% Interface: Ethernet communication Pressure Sensor: 0–20 MPa, accuracy ±0.025 MPa Cleaning: Automatic flushing	Dual-pump gradient system 300 mL/min per pump Supports constant pressure mode and pressure control mode
Mixer	Dynamic mixer: standard 1.4 mL, optional 5 mL or 10 mL	Standard 1.4 mL
UV Detector	Single-wavelength LED light source, Abs: ±6 AU	Single wavelength 280 nm Optional: 190–500 nm, 3 wavelengths Optional: 190–850 nm, 3 wavelengths Optional: 190–850 nm, 4 wavelengths
Conductivity / Temperature Monitor	Conductivity: 0–999 mS/cm Resolution: 0.001 mS/cm Accuracy: ±1.0% Automatic temperature compensation	Standard
pH Valve	pH Range: 0–14 Resolution: 0.01 Accuracy: ±0.05 7-position, 5-port valve pH electrode and restrictor can be online or bypassed	1: Restrictor → pH 2: Restrictor 3: pH 4: Bypass 5: Restrictor
Communication Interface	TCP/IP, 100 Mbps Ethernet	Standard
Accessory Kit	Tubing, fittings and accessories	Standard
Chromatography Workstation Software	Chinese / English user interface, including instrument control, method editing, data processing, report printing, user and result database, encrypted database management, peak integration, quantitative analysis, audit trail, scouting, channel scouting, method programming and 8-channel parallel operation.	Standard
Pump A Inlet Valve	Pump A inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Pump B Inlet Valve	Pump B inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Sample Pump Inlet Valve	Sample pump inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	6 channels + 1 common port high-pressure rotary valve (7 channels)
Air Sensor, Inlet A	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Air Sensor, Inlet B	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Air Sensor, Sample Pump Inlet	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Injection Valve	8-position, 6-port high-pressure rotary valve, pressure rating 30 MPa	1: Manual Load 2: Inject 3: System Pump Waste 4: Sample Pump Load 5: Direct Inject 6: Sample Pump Waste
V Valve	1: 1–2 2: 3–4 3: 1–3 4: 2–4 Pressure rating: 30 MPa	None
Column Valve	Configurable, pressure rating 30 MPa Optional: 2-column valve / 2-column valve with reverse flow / 6-column valve with reverse flow	6-column valve, 5 column flow paths, 1 bypass line
Column Delta Pressure Module	Pre-column pressure, post-column pressure and delta pressure	Standard
Outlet Valve	Maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Back Pressure Valve / Flow Restrictor	HY-FR802	Standard system back pressure: 0.3 MPa
Fraction Collector	1. Frac100A: Open-frame collector, compatible with standard XY100 tubes 2. Frac100B: Enclosed cabinet, 6 rack positions, compatible with 50 mL and 15 mL tubes, automatic rack recognition 3. Frac100B-1: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, automatic rack recognition 4. Frac100B-2: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, with UV sterilization and temperature / humidity monitoring, automatic rack recognition 5. Frac100C: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, with UV sterilization, temperature / humidity monitoring and TE cooling, automatic rack recognition	Frac100B-1
Sample Pump	Flow Rate Range: 0–300 mL/min Maximum Pressure: 10 MPa / 1400 psi Flow Repeatability: 3–300 mL/min, <3 MPa, 0.8–2 cP Flow Accuracy: ±2% Flow Precision: RSD <0.5% Interface: Ethernet communication Pressure Sensor: 0–20 MPa, accuracy ±0.025 MPa Cleaning: Automatic flushing	Standard: 300 mL/min
SDK & Communication Protocol	Optimized proprietary command set. Tested development libraries are provided, including .NET libraries, C/C++ DLLs and sample source code.	Suitable for secondary development.
Human-Machine Interface	Windows 7 / Windows 10 / Windows 11	Configuration: Intel i3 processor or above, memory ≥8 GB, storage ≥256 GB, display resolution 1920 × 1080
Power Supply	110–220 VAC, ±5%
Operating Temperature	-10°C to 40°C, non-condensing

Item	Parameter	Notes
Appearance		(W × D × H) – [mm]: 520 × 440 × 550
System Pump	Flow Rate Range: 0–300 mL/min Maximum Pressure: 2 MPa / 280 psi Flow Repeatability: 6–600 mL/min, <3 MPa, 0.8–2 cP Flow Accuracy: ±2% Flow Precision: RSD <0.5% Gradient Accuracy: ±0.6% Interface: Ethernet communication Pressure Sensor: 0–20 MPa, accuracy ±0.025 MPa Cleaning: Automatic flushing	Dual-pump gradient system 600 mL/min per pump Supports constant pressure mode and pressure control mode
Mixer	Dynamic mixer: standard 1.4 mL, optional 5 mL or 10 mL	Standard 1.4 mL
UV Detector	Single-wavelength LED light source, Abs: ±6 AU	Single wavelength 280 nm Optional: 190–500 nm, 3 wavelengths Optional: 190–850 nm, 3 wavelengths Optional: 190–850 nm, 4 wavelengths
Conductivity / Temperature Monitor	Conductivity: 0–999 mS/cm Resolution: 0.001 mS/cm Accuracy: ±1.0% Automatic temperature compensation	Standard
pH Valve	pH Range: 0–14 Resolution: 0.01 Accuracy: ±0.05 7-position, 5-port valve pH electrode and restrictor can be online or bypassed	1: Restrictor → pH 2: Restrictor 3: pH 4: Bypass 5: Restrictor
Communication Interface	TCP/IP, 100 Mbps Ethernet	Standard
Accessory Kit	Tubing, fittings and accessories	Standard
Chromatography Workstation Software	Chinese / English user interface, including instrument control, method editing, data processing, report printing, user and result database, encrypted database management, peak integration, quantitative analysis, audit trail, scouting, channel scouting, method programming and 8-channel parallel operation.	Standard
Pump A Inlet Valve	Pump A inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Pump B Inlet Valve	Pump B inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Sample Pump Inlet Valve	Sample pump inlet valve, maximum pressure 10 MPa, optional 3 / 7 / 13 channels	6 channels + 1 common port high-pressure rotary valve (7 channels)
Air Sensor, Inlet A	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Air Sensor, Inlet B	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Air Sensor, Sample Pump Inlet	External ultrasonic air sensor	Standard, optional built-in ultrasonic sensor
Injection Valve	8-position, 6-port high-pressure rotary valve, pressure rating 30 MPa	1: Manual Load 2: Inject 3: System Pump Waste 4: Sample Pump Load 5: Direct Inject 6: Sample Pump Waste
V Valve	1: 1–2 2: 3–4 3: 1–3 4: 2–4 Pressure rating: 30 MPa	None
Column Valve	Configurable, pressure rating 30 MPa Optional: 2-column valve / 2-column valve with reverse flow / 6-column valve with reverse flow	6-column valve, 5 column flow paths, 1 bypass line
Column Delta Pressure Module	Pre-column pressure, post-column pressure and delta pressure	Standard
Outlet Valve	Maximum pressure 10 MPa, optional 3 / 7 / 13 channels	12 channels + 1 common port high-pressure rotary valve (13 channels)
Back Pressure Valve / Flow Restrictor	HY-FR802	Standard system back pressure: 0.3 MPa
Fraction Collector	1. Frac100A: Open-frame collector, compatible with standard XY100 tubes 2. Frac100B: Enclosed cabinet, 6 rack positions, compatible with 50 mL and 15 mL tubes, automatic rack recognition 3. Frac100B-1: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, automatic rack recognition 4. Frac100B-2: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, with UV sterilization and temperature / humidity monitoring, automatic rack recognition 5. Frac100C: Enclosed cabinet, 6 rack positions, compatible with 50 mL tubes, 15 mL tubes, 24-well, 48-well and 96-well plates, with UV sterilization, temperature / humidity monitoring and TE cooling, automatic rack recognition	Frac100B-1
Sample Pump	Flow Rate Range: 0–600 mL/min Maximum Pressure: 10 MPa / 1400 psi Flow Repeatability: 6–600 mL/min, <3 MPa, 0.8–2 cP Flow Accuracy: ±2% Flow Precision: RSD <0.5% Interface: Ethernet communication Pressure Sensor: 0–20 MPa, accuracy ±0.025 MPa Cleaning: Automatic flushing	Standard: 600 mL/min
SDK & Communication Protocol	Optimized proprietary command set. Tested development libraries are provided, including .NET libraries, C/C++ DLLs and sample source code.	Suitable for secondary development.
Human-Machine Interface	Windows 7 / Windows 10 / Windows 11	Configuration: Intel i3 processor or above, memory ≥8 GB, storage ≥256 GB, display resolution 1920 × 1080
Power Supply	110–220 VAC, ±5%
Operating Temperature	-10°C to 40°C, non-condensing